Streptavidin sepharose Purification kit
Affinity purified Streptavidin is conjugated to NHS-sepharose. It is an efficient
technique for isolating biotinylated proteins. Streptavidin is a 52.8 kDa protein
purified from the bacterium Streptomyces avidinii. Streptavidin homotetramers
have an extraordinarily high affinity for Biotin.
The Streptavidin sepharose can be used for affinity chromatography purifications, assay development and immunoprecipitation. Streptavidin resins also can be used in the physical separation of two DNA strands produced in a polymerase chain reaction by incorporating biotin in one of the amplification polymers.
technique for isolating biotinylated proteins. Streptavidin is a 52.8 kDa protein
purified from the bacterium Streptomyces avidinii. Streptavidin homotetramers
have an extraordinarily high affinity for Biotin.
The Streptavidin sepharose can be used for affinity chromatography purifications, assay development and immunoprecipitation. Streptavidin resins also can be used in the physical separation of two DNA strands produced in a polymerase chain reaction by incorporating biotin in one of the amplification polymers.
Component:
Note: Sepharose is 1:1 suspension in Phosphate Buffered Saline, pH 7.4, containing 0.05% sodium azide as a preservative.
Product capacity:
The binding and elution capacity of 1 mL settled Streptavidin sepharose are commonly more than 5 mg of biotinylated protein. Trying different elution buffers for optimal results is recommended.
Materials Required but Not Provided:
For sustainable use and long term storage, store at 2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice
| Reagent | Quantity for 10 rxns (C07007-K02) |
Quantity for 5 rxns (C07007-K01) |
composition |
| Streptavidin sepharose |
2 mL X 1 vial | 1 mL X 1 vial | 50% slurry of Streptavidin sepharose in 1X Phosphate Buffered Saline |
| Binding Buffer (10X concentration) | 5.0 mL X 1 vial | 2.5 mL X 1 vial | 200 mM Na2HPO4; 1.5 M NaCl, pH 7.5 |
| Elution Buffer | 10 mL X 1 vial | 5 mL X 1 vial | 0.1 M Glycine pH 2.7 |
| spin column | 10 pcs | 5 pcs | |
| collection tube | 10 tubes | 5 tubes |
Product capacity:
The binding and elution capacity of 1 mL settled Streptavidin sepharose are commonly more than 5 mg of biotinylated protein. Trying different elution buffers for optimal results is recommended.
Materials Required but Not Provided:
- Micro-centrifuge capable of 15,000 x g
- 1.5 mL Centrifuge tubes
- End-over-end rotator
- CoIP Lysis Buffer (mild reaction): 10 mM Tris/Cl pH 7.5; 150 mM NaCl; 0.5 mM EDTA; 0.5% NP-40
- RIPA (vigorous reaction): 100 mM Tris/Cl pH 7.5; 300 mM NaCl; 0.2% Sodium Deoxycholate (or 0.1% SDS); 2% NP-40
- 6 M guanidine-HCl, pH 1.5 for purification of biotinylated molecules
For sustainable use and long term storage, store at 2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice